Improved determination of prostatic acid phosphatase (sodium thymolphthalein monophosphate substrate).
نویسندگان
چکیده
We have modified a previously described method for determining acid phosphatase, with thymolphthalein monophosphate as substrate, to increase its sensitivity. We assessed effects of serum on variables influencing acid phosphatase activity as measured by this method. The method is shown to be not completely specific for prostatic acid phosphatase. The importance of standardizing methodology in measurement of enzyme activities is demonstrated.
منابع مشابه
Effects of substrate concentration on results of determination of prostatic acid phosphatase with thymolphthalein monophosphate.
The relation between concentration of thymolphthalein monophosphate substrate and catalytic activity was investigated for the determination of prostatic acid phosphatase. This study, an extension of previously reported work (Clin. Chem. 27: 1372, 1981), shows that lot-to-lot variation in purity of thymolphthalein monophosphate preparations is reflected in substrate-velocity curves. Plateau regi...
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A new substrate, ammonium thymolphthalein monophosphate, is proposed for use in alkaline and acid phosphatase determinations. The ammonium salt shares the advantages of the sodium salt, but is a more stable substrate and results in more stable working reagents. The substrate is buffered at ph 10.1 in diethanolamine for determining alkaline phosphatase activity in 5 mm, and at pH 5.9 in citrate ...
متن کاملSodium thymolphthalein monophosphate: a new acid phosphatase substrate with greater specificity for the prostatic enzyme in serum.
We describe the use of sodium thymolphthalein monophosphate as a substrate for measuring acid phosphatase activity in serum. Thymolphthalein is liberated in the reaction and is conveniently measured by increasing the pH of the medium, which produces a color and also stops the hydrolysis. Optimal conditionsforenzymatic activitywere defined foruse in the final assay system. Purifiedacid phosphata...
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5. Warren RJ, Moss DW. An automated continuous-monitoring procedure for the determination of acid phosphatase activity in serum. Clin Chim Acta 77, 179-188 (1977). 6. Gundlach G, Muhlhausen B. Untersuchungen zur Kupplung des 1-Naphthols mit Fast-red-TR. J Clin Chem Clin Biochem 18, 603-610 (1980). 7. Babson AL, Read PA. A new assay for prostatic acid phosphatase in serum. Am J Clin Pathol 32, 8...
متن کاملEnhancing effect of surfactant and protein on hydrolysis of thymolphthalein monophosphate by purified prostatic acid phosphatase.
Purified prostatic acid phosphatase catalyzes the hydrolysis of thymolphthalein monophosphate 10-fold faster if an optimal concentration of Brij 35 (a wetting agent) or protein (bovine serum albumin or human serum proteins) is present. Results of gel filtration, dialysis, and sucrose density-gradient centrifugation analysis suggest that the substrate must combine with detergent or protein befor...
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عنوان ژورنال:
- Clinical chemistry
دوره 22 5 شماره
صفحات -
تاریخ انتشار 1976